{"id":113,"date":"2017-10-03T12:59:19","date_gmt":"2017-10-03T05:59:19","guid":{"rendered":"http:\/\/cbg.farmasi.unej.ac.id\/?page_id=113"},"modified":"2017-10-03T12:59:19","modified_gmt":"2017-10-03T05:59:19","slug":"scanometric-methods","status":"publish","type":"page","link":"https:\/\/cbg.farmasi.unej.ac.id\/?page_id=113","title":{"rendered":"Scanometric methods"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">The scanometric method is simply defined as a branch of the colorimetric method that employed a flatbed scanner to obtain an analytical signal by color intensity measurement. Here, we use a paper-based sensor that is simply fabricated by immobilizing antioxidant reagent (e.g. DPPH) onto the circular patterns on the filter paper which acts as the sensing zone, as it can be seen in Video 1. <\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-4-3 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Reagent immobilization in paper-based antioxidant sensor\" width=\"847\" height=\"635\" src=\"https:\/\/www.youtube.com\/embed\/TGNfJo4HLjw?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><figcaption><strong>Video 1<\/strong>. Immobilization of antioxidant reagent onto the sensing zone of the paper based-sensor.<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">In general, the sample solution is transferred onto the sensing zone next to the blank sensing zone as depicted in Video 2. <\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-4-3 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"The addition of sample onto the paper-based sensor.\" width=\"847\" height=\"635\" src=\"https:\/\/www.youtube.com\/embed\/fO6OuTvqAuI?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><figcaption> <strong>Video 2<\/strong>. Addition of sample solution onto the paper based-sensor. <\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">As presented in  Video 3, an ordinary document scanner or flatbed scanner is then used to capture the color change of the sensing zone as a result of the chemical reaction between the sample and immobilized DPPH. <\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-4-3 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Scanning a paper-based antioxidant sensor.\" width=\"847\" height=\"635\" src=\"https:\/\/www.youtube.com\/embed\/FNgTJpO52W8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><figcaption> <strong>Video 3<\/strong>. Scanning the color change of the paper based-sensor after the sample solution addition.  <\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">In a practical sense, the scanned image can be cropped to simplify the color measurement and to reduce the size of the image file as well, as presented in Video 4. <\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Cropping an image of sensing zones\" width=\"847\" height=\"476\" src=\"https:\/\/www.youtube.com\/embed\/0MBrPIL4azM?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><figcaption> <strong>Video 4<\/strong>. Cropping the scanned image of the region of interest of the paper-based sensor.  <\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">The color intensity of the cropped image is then quantified with a color image program (i.e. ImageJ) to generate the average value of red (R), green (G), blue (B), or the combined red\u2013green\u2013blue (RGB) color respectively, as shown in Video 5. For analytical purposes, each color value of the sample addition is subtracted from that of the blank sensing zone.<\/p>\n\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"Color intensity measurement of paper-based sensor\" width=\"847\" height=\"476\" src=\"https:\/\/www.youtube.com\/embed\/FfbxMo92d-M?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><figcaption> <strong>Video 5<\/strong>. The color intensity measurement of the cropped image using ImageJ program.  <\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>The scanometric method is simply defined as a branch of the colorimetric method that employed a flatbed scanner to obtain an analytical signal by color intensity measurement. Here, we use a paper-based sensor that is simply fabricated by immobilizing antioxidant <a href=\"https:\/\/cbg.farmasi.unej.ac.id\/?page_id=113\" class=\"read-more\">Read More &#8230;<\/a><\/p>\n","protected":false},"author":5,"featured_media":0,"parent":26,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-113","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=\/wp\/v2\/pages\/113","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=113"}],"version-history":[{"count":0,"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=\/wp\/v2\/pages\/113\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=\/wp\/v2\/pages\/26"}],"wp:attachment":[{"href":"https:\/\/cbg.farmasi.unej.ac.id\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=113"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}